CEFAS22-154 River water sample collection (PATHSafe)

To collect river water samples (with the potential for collecting water samples from combined sewer overflows during the second phase of the study in summer 2023) in the river Ribble, Lancashire as part of the government's Pathogen Surveillance in Agriculture, Food and the Environment (PATH-SAFE) programme.

Samples will be collected weekly and posted in temperature-controlled boxes by courier to designated laboratories in England.

Sample locations and frequency: Eleven river water samples will be collected weekly from the following locations, subject to accessibility and health and safety requirements.

Site Sample Type Site Details OS grid reference easting OS grid reference northing 1 River water River Darwen - Winery Lane nr A6 355051 428160 7 River water River Douglas - A581 Meadow Lane 346655 417980 8 River water River Ribble - shoreline access under A6 355270 428674 9 River water River Ribble - Dog Hill Brow, east of B6479 381781 467247 10 River water River Hodder - Lower Hodder Bridge, B6243 370433 439194 11 River water River Calder - Bark Park, Ridding Lane, nr.

A59 372342 436338 12 River water River Douglas - pontoon at Douglas Marine 345269 423072 13 River water Ribble estuary - lifeboat station track at Lytham 336737 426665 14 River water Ribble estuary - St Anne's Pier 331699 428502 15 River water SUGGESTED: Ribble Bridge, Brungerley Park 373867 442847 16 River water SUGGESTED: Ribble Bridge, south of Lambing Clough Lane 368550 436636 Sampling duration: Weekly samples will be collected from all sampling locations over a 13-week period between 2nd January and 29th March 2023 and over a 13-week period between 5th June and 30th August 2023.

Sample collection, shipping, and data recording: Sample collection, transport and data recording will be carried out in accordance with the Cefas sampling protocol. https://defra.bravosolution.co.uk/go/447666740184E7E2D8AB